Enzyme-linked Immunosorbent Assay:Article Title: High-throughput engineered tumor organoids reveal ROCK signaling as an immunotherapeutic target in triple-negative breast cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER RPMI Medium 1640 basic(1×) Gibco Cat# C11875500BT QmSuero® Fetal Bovine Serum Qmsuero Cat# mu001SR Mouse Granzymes B (GZMS-B) ELISA Kit Ek-Bioscience Cat# EK-M20626 Mouse Alpha-Smooth Muscle Actin (α-SMA) ELISA kit Ek-Bioscience Cat# EK-M21428 Mouse Phosphorylated Myosin Light Chain (PMLC) ELISA Kit Ek-Bioscience Cat# EK-M21451 R-spondin 1 MCE Cat# HY-P76012 Y-27632 MCE Cat# HY-10583 EGF MCE Cat# HY-P7109 GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Nicotinamide Sigma-Aldrich Cat# N0636 FGF-7 MCE Cat# HY-P7176 FGF 10 MCE Cat# HY-P7170 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 N-Acetylcysteine Sigma-Aldrich Cat# A9165 Noggin (Mouse) MCE Cat# HY-P7086 A83-01 Stem Cell Technologies Cat# 72022 CHIR99021 Stem Cell Technologies Cat# 72052 Red blood cell lysis buffer Roche Cat# 11814389001 Pentanoic acid MCE Cat# HY-N6056 Bovine serum Albumin Sigma-Aldrich Cat# V900933 Sucrose Sigma-Aldrich Cat# S7903 DMSO Sigma-Aldrich Cat# D8418 Matrigel Corning Cat# 354234 Collagenase I Yuanye Cat# S10053 Collagenase IV Yuanye Cat# S10056 Cell Staining Buffer BioLegend Cat# 420201 7-AAD Viability Staining Solution BioLegend Cat# 420404 Chemically Defined Lipid Concentrate (CD lipid) Gibco Cat# 11905031 Glycerol Sigma-Aldrich Cat# G5516 Trypsin Gibco Cat# 25200072 Lithium carbonate Aladdin Cat# L101684-25g IL-2 protein, Mouse MCE Cat# HY-P7077 IL-7 protein, Mouse MCE Cat# HY-P73226 IL-15R alpha Protein, Mouse (HEK293, hFc) MCE Cat# HY-P71978 IL-2 protein, Human MCE Cat# HY-P704698 trans-Vaccenic acid MCE Cat# HY-113427 Dispase II Yeasen Cat# 40104ES60 DNase I Solarbio Cat# 9003-98-9 Compensation beads Biolegend Cat# 424601 Potassium chloride Macklin Cat# P816350 Deposited data Raw sequencing data This paper GSA: CRA035611 Experimental models: Cell lines 4T1 mouse breast cancer cells Procell Cat# CL-0007 (Continued on next page) Cell Reports 45, 116987, February 24, 2026 15 .. The 4T1 mouse breast cancer cells (Procell) were cultured with cancer cell culture medium which included DMEM/F12 (Gibco) with 10% Fetal bovine serum (FBS, Qmsuero) and 1% penicillin-streptomycin solution (P/S, Thermo Fisher Scientific), medium changed for every two days.
Red Blood Cell Lysis:Article Title: High-throughput engineered tumor organoids reveal ROCK signaling as an immunotherapeutic target in triple-negative breast cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER RPMI Medium 1640 basic(1×) Gibco Cat# C11875500BT QmSuero® Fetal Bovine Serum Qmsuero Cat# mu001SR Mouse Granzymes B (GZMS-B) ELISA Kit Ek-Bioscience Cat# EK-M20626 Mouse Alpha-Smooth Muscle Actin (α-SMA) ELISA kit Ek-Bioscience Cat# EK-M21428 Mouse Phosphorylated Myosin Light Chain (PMLC) ELISA Kit Ek-Bioscience Cat# EK-M21451 R-spondin 1 MCE Cat# HY-P76012 Y-27632 MCE Cat# HY-10583 EGF MCE Cat# HY-P7109 GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Nicotinamide Sigma-Aldrich Cat# N0636 FGF-7 MCE Cat# HY-P7176 FGF 10 MCE Cat# HY-P7170 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 N-Acetylcysteine Sigma-Aldrich Cat# A9165 Noggin (Mouse) MCE Cat# HY-P7086 A83-01 Stem Cell Technologies Cat# 72022 CHIR99021 Stem Cell Technologies Cat# 72052 Red blood cell lysis buffer Roche Cat# 11814389001 Pentanoic acid MCE Cat# HY-N6056 Bovine serum Albumin Sigma-Aldrich Cat# V900933 Sucrose Sigma-Aldrich Cat# S7903 DMSO Sigma-Aldrich Cat# D8418 Matrigel Corning Cat# 354234 Collagenase I Yuanye Cat# S10053 Collagenase IV Yuanye Cat# S10056 Cell Staining Buffer BioLegend Cat# 420201 7-AAD Viability Staining Solution BioLegend Cat# 420404 Chemically Defined Lipid Concentrate (CD lipid) Gibco Cat# 11905031 Glycerol Sigma-Aldrich Cat# G5516 Trypsin Gibco Cat# 25200072 Lithium carbonate Aladdin Cat# L101684-25g IL-2 protein, Mouse MCE Cat# HY-P7077 IL-7 protein, Mouse MCE Cat# HY-P73226 IL-15R alpha Protein, Mouse (HEK293, hFc) MCE Cat# HY-P71978 IL-2 protein, Human MCE Cat# HY-P704698 trans-Vaccenic acid MCE Cat# HY-113427 Dispase II Yeasen Cat# 40104ES60 DNase I Solarbio Cat# 9003-98-9 Compensation beads Biolegend Cat# 424601 Potassium chloride Macklin Cat# P816350 Deposited data Raw sequencing data This paper GSA: CRA035611 Experimental models: Cell lines 4T1 mouse breast cancer cells Procell Cat# CL-0007 (Continued on next page) Cell Reports 45, 116987, February 24, 2026 15 .. The 4T1 mouse breast cancer cells (Procell) were cultured with cancer cell culture medium which included DMEM/F12 (Gibco) with 10% Fetal bovine serum (FBS, Qmsuero) and 1% penicillin-streptomycin solution (P/S, Thermo Fisher Scientific), medium changed for every two days.
Staining:Article Title: High-throughput engineered tumor organoids reveal ROCK signaling as an immunotherapeutic target in triple-negative breast cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER RPMI Medium 1640 basic(1×) Gibco Cat# C11875500BT QmSuero® Fetal Bovine Serum Qmsuero Cat# mu001SR Mouse Granzymes B (GZMS-B) ELISA Kit Ek-Bioscience Cat# EK-M20626 Mouse Alpha-Smooth Muscle Actin (α-SMA) ELISA kit Ek-Bioscience Cat# EK-M21428 Mouse Phosphorylated Myosin Light Chain (PMLC) ELISA Kit Ek-Bioscience Cat# EK-M21451 R-spondin 1 MCE Cat# HY-P76012 Y-27632 MCE Cat# HY-10583 EGF MCE Cat# HY-P7109 GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Nicotinamide Sigma-Aldrich Cat# N0636 FGF-7 MCE Cat# HY-P7176 FGF 10 MCE Cat# HY-P7170 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 N-Acetylcysteine Sigma-Aldrich Cat# A9165 Noggin (Mouse) MCE Cat# HY-P7086 A83-01 Stem Cell Technologies Cat# 72022 CHIR99021 Stem Cell Technologies Cat# 72052 Red blood cell lysis buffer Roche Cat# 11814389001 Pentanoic acid MCE Cat# HY-N6056 Bovine serum Albumin Sigma-Aldrich Cat# V900933 Sucrose Sigma-Aldrich Cat# S7903 DMSO Sigma-Aldrich Cat# D8418 Matrigel Corning Cat# 354234 Collagenase I Yuanye Cat# S10053 Collagenase IV Yuanye Cat# S10056 Cell Staining Buffer BioLegend Cat# 420201 7-AAD Viability Staining Solution BioLegend Cat# 420404 Chemically Defined Lipid Concentrate (CD lipid) Gibco Cat# 11905031 Glycerol Sigma-Aldrich Cat# G5516 Trypsin Gibco Cat# 25200072 Lithium carbonate Aladdin Cat# L101684-25g IL-2 protein, Mouse MCE Cat# HY-P7077 IL-7 protein, Mouse MCE Cat# HY-P73226 IL-15R alpha Protein, Mouse (HEK293, hFc) MCE Cat# HY-P71978 IL-2 protein, Human MCE Cat# HY-P704698 trans-Vaccenic acid MCE Cat# HY-113427 Dispase II Yeasen Cat# 40104ES60 DNase I Solarbio Cat# 9003-98-9 Compensation beads Biolegend Cat# 424601 Potassium chloride Macklin Cat# P816350 Deposited data Raw sequencing data This paper GSA: CRA035611 Experimental models: Cell lines 4T1 mouse breast cancer cells Procell Cat# CL-0007 (Continued on next page) Cell Reports 45, 116987, February 24, 2026 15 .. The 4T1 mouse breast cancer cells (Procell) were cultured with cancer cell culture medium which included DMEM/F12 (Gibco) with 10% Fetal bovine serum (FBS, Qmsuero) and 1% penicillin-streptomycin solution (P/S, Thermo Fisher Scientific), medium changed for every two days.
Sequencing:Article Title: High-throughput engineered tumor organoids reveal ROCK signaling as an immunotherapeutic target in triple-negative breast cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER RPMI Medium 1640 basic(1×) Gibco Cat# C11875500BT QmSuero® Fetal Bovine Serum Qmsuero Cat# mu001SR Mouse Granzymes B (GZMS-B) ELISA Kit Ek-Bioscience Cat# EK-M20626 Mouse Alpha-Smooth Muscle Actin (α-SMA) ELISA kit Ek-Bioscience Cat# EK-M21428 Mouse Phosphorylated Myosin Light Chain (PMLC) ELISA Kit Ek-Bioscience Cat# EK-M21451 R-spondin 1 MCE Cat# HY-P76012 Y-27632 MCE Cat# HY-10583 EGF MCE Cat# HY-P7109 GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Nicotinamide Sigma-Aldrich Cat# N0636 FGF-7 MCE Cat# HY-P7176 FGF 10 MCE Cat# HY-P7170 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 N-Acetylcysteine Sigma-Aldrich Cat# A9165 Noggin (Mouse) MCE Cat# HY-P7086 A83-01 Stem Cell Technologies Cat# 72022 CHIR99021 Stem Cell Technologies Cat# 72052 Red blood cell lysis buffer Roche Cat# 11814389001 Pentanoic acid MCE Cat# HY-N6056 Bovine serum Albumin Sigma-Aldrich Cat# V900933 Sucrose Sigma-Aldrich Cat# S7903 DMSO Sigma-Aldrich Cat# D8418 Matrigel Corning Cat# 354234 Collagenase I Yuanye Cat# S10053 Collagenase IV Yuanye Cat# S10056 Cell Staining Buffer BioLegend Cat# 420201 7-AAD Viability Staining Solution BioLegend Cat# 420404 Chemically Defined Lipid Concentrate (CD lipid) Gibco Cat# 11905031 Glycerol Sigma-Aldrich Cat# G5516 Trypsin Gibco Cat# 25200072 Lithium carbonate Aladdin Cat# L101684-25g IL-2 protein, Mouse MCE Cat# HY-P7077 IL-7 protein, Mouse MCE Cat# HY-P73226 IL-15R alpha Protein, Mouse (HEK293, hFc) MCE Cat# HY-P71978 IL-2 protein, Human MCE Cat# HY-P704698 trans-Vaccenic acid MCE Cat# HY-113427 Dispase II Yeasen Cat# 40104ES60 DNase I Solarbio Cat# 9003-98-9 Compensation beads Biolegend Cat# 424601 Potassium chloride Macklin Cat# P816350 Deposited data Raw sequencing data This paper GSA: CRA035611 Experimental models: Cell lines 4T1 mouse breast cancer cells Procell Cat# CL-0007 (Continued on next page) Cell Reports 45, 116987, February 24, 2026 15 .. The 4T1 mouse breast cancer cells (Procell) were cultured with cancer cell culture medium which included DMEM/F12 (Gibco) with 10% Fetal bovine serum (FBS, Qmsuero) and 1% penicillin-streptomycin solution (P/S, Thermo Fisher Scientific), medium changed for every two days.
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